Antibacterial activities of some borneo plant extracts. Antibacterial australian plants griffith university. The results revealed that the extracts of the two plant species caused a considerable reduction in the number of weevils. Culicidae samuel tennyson1, k john ravindran2, s arivoli3 1department of zoology, madras christian college, 600 059, tamilnadu, india 2national institute of malaria research icmr, field unit chennai, national institute of epidemiology campus, chennai 600. Abstract several plant species were selected as potentially safer substitutes for control of cowpea weevil, callosobruchus maculatus. In the present study, the plant extracts exhibited the presence of larvicidal activity table 1 which was a dose dependent effect. Abdul hakeem college, melvisharam 632 509, vellore district, tamil nadu, india contents lists available at. Pdf nematicidal activity of plant extracts against the root. The present study was carried out to evaluate the adulticidal, repellent, and larvicidal.
Efficacy of plant extracts in plant disease management. Studies on some plant extracts for their antimicrobial. Biochemistry graduates to have results from their final year research projects on the antibacterial activity of plant extracts submitted for publication dilruba meah and aiysha thompson have just finished their biochemistry. Many plant extracts and essential oils have been screened for their nematicidal activity against the plant parasitic nematodes814. Based on the preliminary screening, the effective plant extracts were further tested at a concentration of 0.
In the previous chapters 3 and 4, it was shown that p. S1 document heading screening of twenty five plant extracts for larvicidal activity against culex quinquefasciatus say diptera. Spodoptera litura, plant extract mixture, synergist, detoxification. Pdf larvicidal activity of plant extracts on aedes aegypti l. Evaluation of larvicidal activity of medicinal plant. Methods petroleum ether, chloroform and aqueous extracts obtained from acalypha indica, aerva lanata, boerhaavia. Pavela20 studied the larvicidal activity of thirty one euroasiatic methanolic plant extracts against the larvae of culex quinquefasciatus. The leaf extracts of romatic plants, murraya koenigii and azadirachta a. The activity against 15 isolates of each bacterium was determined by disc diffusion method at a concentration of 5%.
The present study aimed to assess the cytotoxic activity of extracts of selected plant species used for treatment of malaria in tanzania. Since, the both plants are economically safe and less expensive to control mosquito and the histological studies to be performed to recognize the mode of action between leaf extract and mosquito. The objective of this study was to determine the chemical composition and antibacterial activity of essential oils eos of tagetes minuta against three phytopathogenic bacteria pseudomonas savastanoi pv. The number of eggs hatched in control and treated were recorded and percentage of ovicidal activity calculated. Plants are collected either randomly or by following leads supplied by local healers in geographical areas where the plants are found. Antioxidant activity of plant extracts containing phenolic. In the present study, the ovicidal effect of twenty five plants screened against spodoptera litura at 0. Juss were evaluated for their growth, adult mortality and oviposition inhibition of c. Review cytotoxic studies of plant extracts cytotoxicity of plant extracts on both plant and animal test material is capturing scientific interest, as it can indicate their antitumor and anticarcinogenic potentials, which have always been a gateway for the development of novel anticancer drugs. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Antioxidant properties of the methanol extracts of the leaves. Fresh or dried plant materials can be used as a source for the extraction of secondary.
The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. Many plant extracts and essential oils have been screened for their nematicidal activity against the plantparasitic nematodes814. Such results were different from data reported in literature. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. Evaluation of the larvicidal efficacy of five indigenous weeds.
Phytochemical investigation and biological activity of leaves. Nematicidal activity of plant extracts against the rootknot nematode, meloidogyne incognita article pdf available in the open natural products journal 21 october 2009 with 2,550 reads. Methanol extracts of the plant was obtained by taking 50g of dried leaf and flower. Larvicidal activity of some plant extracts against mosquitoes. Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibioticresistant bacteria. Pdf nematicidal activity of plant extracts against the. Pdf larvicidal activity of plant and algae extracts. Antisnake venom activity of different extracts of pouzolzia indica against russel viper venom anas ahmed1, k. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively. Debnath department of pharmaceutical sciences, dibrugarh university, dibrugarh 786 004, india. Antifeedant activity of plant extracts to an insect helopeltis theivora a. In vitro antibacterial activity of several plant extracts.
The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. Plant biology and biotechnology, cah college, melvisharam, tamil nadu, india. Biochemistry graduates to have results from their final year. Pdf to evaluate the larvicidal and repellent activities of ethyl acetate and. Ovicidal activity of plant extracts against litura. Neem leaf extract the leaves of the plant material were shade dried 282c, ground and sieved to get fine powder from which the extracts were prepared. Larvicidal bioassay the larvicidal activity of crude extracts of the selected plants was assessed by the protocol of who15 with some modifications and as per the method of rahuman et a16. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay. All the three solvent extracts of the plant parts possess major bioactive compounds that are responsible for many pharmacological activities being reported. The activity of crude plant extracts is often attributed to the complex mixture of active compounds. Chemical composition and antibacterial activity of essential. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material.
Screening of in vitro antioxidant activity of methanolic. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. Mosquitoes larvicidal activity of leaf extract of neem azadirachta indica. For the bioassay in a container 25 fourth instar larvae were kept in 249 ml of distilled water with 1 ml of extracts 400 ppm in dmso. The larvicidal activity of medicinal plant extracts were tested against early. Larvicidal activity of some plant extracts against mosquitoes dr. The chemical examination follows after the isolation and biological screening of the active fraction1. Larvicidal activity of medicinal plant extracts against. Institute has screened about 35,000 higher plant species for activity against cancer, where about 3,000 of these.
Larvicidal and pupicidal effect of ethanolic extract of. Antioxidant and in vitro cytotoxic activity of extracts of. Antifeedant activity of plant extracts to an insect. Medicinal plants, ethanol, methanol and aqueous extracts, human pathogens, antimicrobial activity. Antimicrobial activity of crude extracts of three medicinal. Larvicidal activity of selected plant extracts against the. Screening of some plants in egypt for their cytotoxicity. Ovicidal activity of plant extracts is important to control the pest at egg stage itself thereby preventing the damage caused by other stages 25. Issn larvicidal activity of monstera adansonii plant. In the preliminary screening, potential larvicidal activity of plants, different solvents crude extracts of three plants was noted table ii, fig. All plant extracts showed significant larvicidal activity against a. Larvicidal activity of some plant extracts against two mosquito.
Jain5 1department of pharmacognosy, mcops, manipal576104, india. Antioxidant properties of the methanol extracts of the. Assessment of antioxidant activity of plant extracts by. Evaluation of nematicidal activity of ethanol extracts of. In vitro antibacterial activity of several plant extracts and. The effect of various plant extracts was measured on several important oxidation indicators. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. The plant extracts were screened for larvicidal activity individually and in combination against the larvae of aedes aegypti and culex quinquefasciatus as per who protocol. Evaluation of the larvicidal efficacy of five indigenous. Ethanol extracts were evaluated for cytoxicity by using mtt assay on llcmk2 cells and by brine shrimp lethality assay. Larvicidal activity of plant and algae extracts, essential oils and isolated chemical constituents against aedes aegypti article pdf available january 20 with 855 reads how we measure reads. A plantbased extract mixture for controlling spodoptera litura. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method.
The high larvicidal activity of tinospora rumphii leaf is supported by the abundance of phytochemicals which show synergestic effects in terms of larvicidal action to mosquito larvae. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. Pdf larvicidal and repellent activity of medicinal plant extracts. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. Euphorbia fischeriana steud is a perennial herbaceous plant with latex, distributed mainly in north china 15. Screening of some plants in egypt for their cytotoxicity against four human cancer cell lines. Antibacterial activity of plants 719 effective extracts are considered, e. Antibacterial activity of aqueous extract of calotropis. Fresh leaves of eleven different plant species free from.
Biochemistry graduates to have results from their final. Pdf larvicidal synergistic activity of plant extracts. Bioactive aqueous extracts of twelve yucatecan plants. Plant extracts were prepared according to the procedure described by 10 with slight modification.
Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. In recent years, use of environment friendly and biodegradable natural insecticides of plant origin have received renewed attention as agents for vector control because they are rich in bioactive chemicals, active against a limited number of species including specific target insects, and biodegradable. In the present study, we evaluated the potential of 5 selected plants to inhibit test bacteria by agar well diffusion assay and the result is shown in table 2. Phytochemical investigation and biological activity of. Pdf larvicidal synergistic activity of plant extracts for. These results show that the aqueous extracts of five native plants of. Some compound mixtures increased the glutathionestransferase activities in vivo. Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Antimicrobial activity of crude extracts of some plant leaves. Evaluation of larvicidal activity of medicinal plant extracts against three mosquito vectors a bagavan, a abdul rahuman unit of nanotechnology and bioactive natural products, post graduate and research department of zoology, c.
Antioxidant activity was studied using dpph, cuprac, reducing power assay, deoxyribose degradation site and nonsite specific, ferric reducing antioxidant potential frap, ferric thiocyanate ftc. Screening of twenty five plant extracts for larvicidal. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Antimicrobial activity of some plant extracts against. Therefore, the antimicrobial activity of plant extracts depends on the species of plant, the type of solvent and the type of tested microorganism. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether. In the present study, methanol and aqueous extracts of parkinsonia aculeata l.
The plan of this work was to study the larvicidal activity of cassia. Effect of monstera adansonii on 4th instar larvae of culex quinquefasciatus. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Antibacterial activity of crude extracts of selected plants. Insecticidal and larvicidal activities of medicinal plant. Rajendaran1, durga jaiswal2, hari pratap singh3, ashutosh mishra3, dinesh chandra4, indranil kumar yadav5and d.
In order to extract different phenolic compounds from plants with a high degree. Madhura mukadam department of zoology, gogate jogalekar college, ratnagiri, maharastra, india abstractmosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis. Antimicrobial activity of selected medicinal plants against. Antimicrobial activity of selected medicinal plants. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. Objective to evaluate the larvicidal activity of plant extracts on aedes aegypti. Biologically active plant extracts have been well recognized for.
Likewise, sakthivadivel and daniel21 screened petroleum ether extracts of sixty three insecticidal plants for larvicidal activity against cx. In general, all the tested microorganisms were inhibited by several plant extracts of different solvents used in this study. Antioxidant, antihypertensive, antihyperglycemic, and. Evaluation of the cytotoxic activity of extracts from.
Insecticide repellency of plant extracts against callosobruchus maculatus obadofin, a. Results of antimicrobial activity of the five plant extracts can suggested that s. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. Materials and methods plant species and sample preparation.
Larvicidal activity of plant extracts used alone and in. Quality of plant extract depends on plant material, choice of solvents and the extraction methods. The methanolic extract of caryophyllus aromaticus presented the highest antis. Pdf of full length paper is available online introduction tea is a gift of. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29. By ngaruiya paul njuguna a thesis submitted in partial fulfilment of master of. Screening of in vitro antioxidant activity of methanolic leaf.
The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Phytochemical, fluorescence screening and gcms analysis of. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated as abts radical scavenging activity % abs control. Antibacterial activity of plant extracts and phytochemicals. Pdf larvicidal activity of selected plant extracts and. Plant extracts 1 ml were allowed to react with 1 ml of the abts solution and the absorbance was taken at 734 nm after 7 min using the spectrophotometer. Cocka,b abiomolecular and physical sciences, logan campus, griffith university, university drive, meadowbrook, queensland 41, australia bgenomics research centre, gold coast campus, griffith university, parklands drive, southport, queensland 4222, australia.